Residual activity was mainly zinc ion-dependent matrix metalloproteinase (MMP) activity (18 ± 6.4%). ![]() TGA subanalysis showed that the majority of the activity was NE (82 ± 6.4%). NCFB patients showed significantly higher neutrophils, CXCL8, TNF-α, NE and TGA than healthy controls. The computed tomography (CT) closest to the date sputum induction was scored by a radiologist. Patient files were analysed to determine Pseudomonas aeruginosa colonization status. Each patient was evaluated by spirometry, Leicester Cough Questionnaire (LCQ) and Sputum Colour Chart (SCC). Sputum was analysed for total and differential cell count, markers of inflammation (CXCL8 (also known as interleukin-8) and tumour necrosis factor-α (TNF-α)) and proteolytic enzymes (neutrophil elastase (NE), gelatin zymography and total gelatinolytic activity (TGA)). ![]() ![]() ![]() Sputum induction in NCFB patients and healthy controls was performed. Our aim was to relate the value of proteolytic enzymes, proteolytic enzyme activity and inflammatory markers to disease severity and symptoms in patients with NCFB. Non-cystic fibrosis bronchiectasis (NCFB) is characterized by a vicious cycle of airway infection, inflammation and structural damage with inappropriate mucus clearance.
0 Comments
Leave a Reply. |